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[pronut-hiv] Inactivation of HIV-1 in breast milk using sodium dodecyl sulfate


  • From: "ProNut-HIV" <pronut-hiv@healthnet.org>
  • Date: Thu, 24 Aug 2006 04:54:03 -0400

From: [PMTCTforum]
Inactivation of HIV-1 in breast milk by treatment with the alkyl sulfate microbicide sodium dodecyl sulfate (SDS)

Sandra Urdaneta, Brian Wigdahl, Elizabeth B Neely, Cheston M Berlin Jr, Cara-Lynne Schengrund, Hung-Mo Lin and Mary K Howett Retrovirology 2005,2:28

doi:10.1186/1742-4690-2-28

The electronic version of this article is open access and can be found online at: http://www.retrovirology.com/content/2/1/28

Abstract

Background: Reducing transmission of HIV-1 through breast milk is needed to help decrease the burden of pediatric HIV/AIDS in society. We have previously reported that alkyl sulfates (i.e., sodium dodecyl sulfate, SDS) are microbicidal against HIV-1 at low concentrations, are biodegradable, have little/no toxicity and are inexpensive. Therefore, they may be used for treatment of HIV-1 infected breast milk. In this report, human milk was artificially infected by adding to it HIV-1 (cell-free or cell-associated) and treated with *1% SDS (*10 mg/ml). Microbicidal treatment was at 37°C or room temperature for 10 min. SDS removal was performed with a commercially available resin. Infectivity of HIV-1 and HIV-1 load in breast milk were determined after treatment.

Results: SDS (*0.1%) was virucidal against cell-free and cell-associated HIV-1 in breast milk. SDS could be substantially removed from breast milk, without recovery of viral infectivity. Viral load in artificially infected milk was reduced to undetectable levels after treatment with 0.1% SDS. SDS was virucidal against HIV-1 in human milk and could be removed from breast milk if necessary. Milk was not infectious after SDS removal.

Conclusion: The proposed treatment concentrations are within reported safe limits for ingestion of SDS by children of 1 g/kg/day. Therefore, use of alkyl sulfate microbicides, such as SDS, to treat HIV1-infected breast milk may be a novel alternative to help revent/reduce transmission of HIV-1 through breastfeeding.